Amino Acid Substituted Resins from AAPPTec

AAPPTec offers an extensive selection of amino acid substituted resins, each manufactured to fall within the 0.4 to 0.8 mmol/g substitution range. For more information about amino acid substituted resins and other AAPPTec resins for peptide synthesis, click here.

One problem often encountered in preparing amino acid substituted resins is incomplete reaction of all the reactive sites on the resin. Unless they are blocked, these unreacted sites can generate impurities that may be difficult to remove. This is especially important in preparing libraries. In combinatorial libraries, impurities can interfere in the library screening. Parallel libraries often are too large to allow practical purification of each library compound, therefore reducing the chances for impurities to form requires well-capped amino acid substituted resins. Inefficient attachment of the protected amino acid also lowers the useful substitution of the amino acid substituted resin, reducing the overall yield and efficiency of the solid phase synthesis. Any unreacted sites in AAPPTec amino acid substituted resins are capped to prevent the formation of truncated peptide impurities.

Partial racemization of the protected amino acids is another problem that can occur while preparing amino acid substituted resins. Numerous catalysts, activating agents, additives and reaction conditions have been tested to find the optimum conditions for preparing amino acid substituted resins. As may be expected, these conditions vary for the different resins. AAPPTec amino acid substituted resins are prepared by proven procedures that ensure good substitution and minimize racemization of the substrates. Each amino acid substituted resin is end-capped to block any remaining unreacted active resin sites that could interfere later in the synthesis.

RWI101